The FlyPi, an all-in-one biology lab

With 3D printed and electronic components, the FlyPi is an affordable modular system that can be used for different imaging experiments including optical and fluorescence microscopy, calcium imaging, optogenetics, thermogenetics and behavioural tracking.

Due to its structure, modules can be individually  fitted to the  system so the same device can be used in different settings and experiments. Moreover, new modules can be created by the users allowing them to come up with new and custom experiments to answer their own scientific questions.

Depending on the application (photos or video) the image resolution of the high-resolution camera can be adjusted either for fast acquisition (up to 90 frames per second) or for high image resolution (2592 x 1944 pixels).

Through a Raspberry Pi computer board, the FlyPi can be connected to the Internet. This technological innovation and the possibility to use the FlyPi in battery mode allows users to collect data anywhere in the world and send it to another location in a timely manner.

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Assembled FlyPi V1.0

Furthermore, the system has been published in PLOS Biology, an Open Access high impact peer-reviewed journal. You can read it here (

If we have sparked your interest in the FlyPi, please contact us via the form on the contact page. There you have the opportunity to register as an "early adopter" and / or to sign up for the mailing list.

If you have already built a Flypi, or if you are currently using one, head over to the community Forum!

Light microscopy - The FlyPi is appropriate to provide high-resolution video of zebrafish larvae (Danio rerio) with only room lighting.
Fluorescence microscopy - Three days postfertilisation (dpf) Zebrafish larva expressing GCaMP5Gf in neurons (HuC:GCaMP5G) in transmission (left) and fluorescence mode (right).
Optogenetic - Drosophila larvae expressing ChR2 in all neurons (elav-GAL4/+; UAS-shibrets; UAS-ChR2/+; UAS-ChR2/+) crawling on ink-stained agar reliably contract when blue LEDs are active.

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